Golden Gate method enables fully-synthetic engineering of therapeutically relevant bacteriophages
Phys.org • • health
Key Points:
- Researchers from New England Biolabs (NEB) and Yale University have developed the first fully synthetic bacteriophage engineering system targeting Pseudomonas aeruginosa, an antibiotic-resistant bacterium, using NEB's High-Complexity Golden Gate Assembly (HC-GGA) platform.
- This new method allows bacteriophages to be engineered synthetically from digital sequence data rather than physical isolates, enabling rapid genome assembly from synthetic DNA fragments with programmed modifications such as host range alteration and fluorescent infection reporters.
- The Golden Gate Assembly technique simplifies bacteriophage engineering by assembling numerous short DNA segments, reducing toxicity and errors compared to traditional methods, and bypasses the need for labor-intensive screening or specialized host strains.
- The collaboration between NEB
