The molecular basis of force selectivity by PIEZO2
Nature • • science
Key Points:
- The study utilized MINFLUX 3D microscopy, electrophysiology, cross-linking mass spectrometry, and genetic manipulation to investigate mouse PIEZO1 and PIEZO2 ion channels, with experiments repeated multiple times and controls included for specificity.
- Mouse PIEZO2 and PIEZO1 constructs were codon-optimized and tagged for imaging and electrophysiology, with specific mutations and chimeras created to study structural and functional aspects, verified by sequencing.
- PtK2 and HEK293 cells were prepared with embedded gold fiducials for MINFLUX imaging and transfected with PIEZO constructs; treatments included osmotic stimulation and cytoskeletal inhibitors to assess channel behavior under different conditions.
- MINFLUX data analysis involved advanced clustering
